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human liver cancer cell line hepg2  (ATCC)


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    Structured Review

    ATCC human liver cancer cell line hepg2
    Human Liver Cancer Cell Line Hepg2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 30028 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+liver+cancer+cell+line/Hep+G2/pm42265110-297-1-8
    Average 99 stars, based on 30028 article reviews
    human liver cancer cell line hepg2 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Discovery of Spirosnuolides A–D, Type I/III Hybrid Polyketide Spiro-Macrolides for a Chemotherapeutic Lead against Lung Cancer
    Article Snippet: After adding SrtA, the fluorescence intensity was measured using a microplate reader (FLx800, BioTek Instruments, Winooski, VT, USA) at excitation and emission wavelengths of 320 nm and 420 nm, respectively. .. Cell culture and chemicals Human lung cancer cell line (A549, HCC827, H1975, H1299), human breast cancer cell line (MDA-MB-231), human colon cancer cell line (HCT116), and human liver cancer cell line (SK-Hep-1) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. Human gastric cancer cell line (SNU-638) and lung epithelial normal cell line (MRC-5) were purchased from the Korean Cell Line Bank (Seoul, Korea).

    Article Title: Application of Helmholtz Coils and Superparamagnetic Nanocarrier in Localized Inhibition of Cancer Cell Migration and Invasion
    Article Snippet: Patients with advanced hepatocellular carcinoma (HCC) have a high death rate, and sorafenib, which has low solubility, is a common drug for HCC treatment.. A system was designed to increase the bioavailability of sorafenib: sorafenib was loaded into iron oxide nanoparticles and extremely low frequency electromagnetic field (EMF; 100 Hz, 5mT) was applied to enhance sorafenib release from the nanocarrier.. FTIR spectrum of the nanocarrier confirmed the existence of alginate coating and sorafenib.

    Multiple Displacement Amplification:

    Article Title: Discovery of Spirosnuolides A–D, Type I/III Hybrid Polyketide Spiro-Macrolides for a Chemotherapeutic Lead against Lung Cancer
    Article Snippet: After adding SrtA, the fluorescence intensity was measured using a microplate reader (FLx800, BioTek Instruments, Winooski, VT, USA) at excitation and emission wavelengths of 320 nm and 420 nm, respectively. .. Cell culture and chemicals Human lung cancer cell line (A549, HCC827, H1975, H1299), human breast cancer cell line (MDA-MB-231), human colon cancer cell line (HCT116), and human liver cancer cell line (SK-Hep-1) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. Human gastric cancer cell line (SNU-638) and lung epithelial normal cell line (MRC-5) were purchased from the Korean Cell Line Bank (Seoul, Korea).

    Mouse Assay:

    Article Title: Analogues of oleuropein and oleacein and uses thereof
    Article Snippet: .. Effect of GS27 on HepG2 Liver Cancer Tumor Growth in Mice The effect of GS27 on liver cancer tumor growth in mice was evaluated in a HepG2 xenograft mouse model. HepG2 is a human liver cancer cell line, obtained from ATCC (cat. No HB-8065). ..

    Modification:

    Article Title: Electromagnetic wireless remote control of mammalian transgene expression
    Article Snippet: .. Human embryonic kidney cells (HEK-293, ATCC, CRL-11268), human telomerase-immortalized mesenchymal stem cells (hMSC-TERT, RRID: CVCL_Z015), human liver cancer cell line (HepG2, ATCC, CRL-11997), Chinese hamster ovary cells (CHO-K1, ATCC, CCL-61), baby hamster kidney cells (BHK-21, ATCC, CCL-10) and mouse pituitary tumour cells (AtT-20, ATCC, CCL-89), were cultivated in Dulbecco’s modified Eagle’s medium (DMEM, catalogue number 52100-39, Thermo Fisher Scientific) supplemented with 100 mM proline (CHO-K1 only), 10% fetal bovine serum (FBS, catalogue number F7524, Sigma-Aldrich) and 1% (v/v) streptomycin/penicillin (catalogue number L0022, Biowest) at 37 °C in a humidified atmosphere containing 5% CO 2 . .. For transfection, 10 4 cells (CellDrop BF Brightfield Cell Counter, DeNovix) were seeded per well in a 96-well plate (catalogue number 3599, Corning Life Sciences) 24 h before transfection by addition of 20 μl of a mixture containing 0.3 μg polyethyleneimine (PEI MAX, mol. wt 40,000, 1 μg μl −1 in double-distilled H 2 O, catalogue number 24765-2, Polysciences) and 0.1 μg plasmid DNA (equimolar concentrations for plasmid mixtures) per well.

    Article Title: LUC7L2 accelerates the growth of liver cancer cells by enhancing DNA damage repair via RRAS.
    Article Snippet: Background & objectives: LUC7L2 may be involved in the recognition of non-consensus splice donor sites in association with the U1 snRNP spliceosomal subunit.. However, their detailed features and regulatory mechanisms of LUC7L2 in the development of human liver cancer have not been well characterized.. Results: Herein, our results demonstrate that LUC7L2 promotes the proliferation of liver cancer cells in vitro and xenograft transplantation in vivo.

    Article Title: Electromagnetic wireless remote control of mammalian transgene expression.
    Article Snippet: .. Human embryonic kidney cells (HEK-293, ATCC, CRL11268), human telomerase-immortalized mesenchymal stem cells (hMSC-TERT, RRID: CVCL_Z015), human liver cancer cell line (HepG2, ATCC, CRL-11997), Chinese hamster ovary cells (CHO-K1, ATCC, CCL-61), baby hamster kidney cells (BHK-21, ATCC, CCL-10) and mouse pituitary tumour cells (AtT-20, ATCC, CCL-89), were cultivated in Dulbecco’s modified Eagle’s medium (DMEM, catalogue number 52100-39, Thermo Fisher Scientific) supplemented with 100 mM proline (CHO-K1 only), 10% fetal bovine serum (FBS, catalogue number F7524, Sigma-Aldrich) and 1% (v/v) streptomycin/penicillin (catalogue number L0022, Biowest) at 37 °C in a humidified atmosphere containing 5% CO2. ..

    Electrophoresis:

    Article Title: Synthesis, Anti-Tumour, and Antibacterial Activities of Monocarbonyl Curcumin Analogues of Piperidones
    Article Snippet: .. Human liver cancer cell line (HepG2, ATCC ® HB-8065TM), human cervical cancer cell line (HeLa, ATCC ® CCL-2TM), human breast cancer cell line (MCF-7, ATCC ® HTB-22TM), human non-small cell lung cancer cell line (A549, ATCC ® CCL-185), and human normal liver cell line (L02, ATCC ® CRL-1417) were all purchased from Peking Union Medical College cell Bank; fetal bovine serum (Batch number: 2022057), PBS buffer (Batch number: 2042168), DMEM High sugar culture medium (Batch number: 2042166), and DMEM Low sugar culture medium (Batch number: 2030101) were all purchased from Biological Industries Tel Aviv, Israel; the 0.25% trypsin EDTA (Batch number: CR2012104), Penicillomycin mixture (Batch number: HJ202305 ), Annexin V and PI Apoptosis Detection Kit (Batch number: HY0821, Manufacturer: Biorigin (Beijing, China) Inc.), RIPA lysate (Batch number: G2002), GAPDH (Batch Number: GB12002), Electrophoresis buffer (Batch number: G2018), and TBS buffer (Batch number: G0001-2L) were all purchased from Wuhan Servicebio Technology Co., Ltd Wuhan, China. .. ; Dimethyl sulfoxide DMSO (Batch number: Z28N11Y132686, Manufacturer: Shanghai Yuanye Biology Shanghai, China); MTT (Batch number: 19110601), and Cell Counting Kit-8 (Batch Number: DCM7126) were all purchased from Beijing Lablead Trading Co., Ltd., Beijing, China.



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    ATCC hepg2 hb 8065 human liver cancer cell lines
    Porphyrin-LNP synthesis and characterization. (A) Porphyrin-LNPs were synthesized via microfluidic rapid mixing. (B) . Cryo-TEM image of porphyrin-LNP (scale bar = 100 nm). (C) Size distribution of porphyrin-LNP-si-Scramble and (D) porphyrin-LNP-si-CTNNB1 measured by dynamic light scattering (DLS). (E) Confocal imaging of FAM-siRNA delivery by porphyrin-LNP. Hep3B and <t>HepG2</t> cells were treated with porphyrin-LNP encapsulating FAM-siRNA (200 nM, ~2 μM porphyrin) for 6 h. Confocal images show FAM-siRNA (green) and porphyrin (magenta).
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    Porphyrin-LNP synthesis and characterization. (A) Porphyrin-LNPs were synthesized via microfluidic rapid mixing. (B) . Cryo-TEM image of porphyrin-LNP (scale bar = 100 nm). (C) Size distribution of porphyrin-LNP-si-Scramble and (D) porphyrin-LNP-si-CTNNB1 measured by dynamic light scattering (DLS). (E) Confocal imaging of FAM-siRNA delivery by porphyrin-LNP. Hep3B and <t>HepG2</t> cells were treated with porphyrin-LNP encapsulating FAM-siRNA (200 nM, ~2 μM porphyrin) for 6 h. Confocal images show FAM-siRNA (green) and porphyrin (magenta).
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    Image Search Results


    Porphyrin-LNP synthesis and characterization. (A) Porphyrin-LNPs were synthesized via microfluidic rapid mixing. (B) . Cryo-TEM image of porphyrin-LNP (scale bar = 100 nm). (C) Size distribution of porphyrin-LNP-si-Scramble and (D) porphyrin-LNP-si-CTNNB1 measured by dynamic light scattering (DLS). (E) Confocal imaging of FAM-siRNA delivery by porphyrin-LNP. Hep3B and HepG2 cells were treated with porphyrin-LNP encapsulating FAM-siRNA (200 nM, ~2 μM porphyrin) for 6 h. Confocal images show FAM-siRNA (green) and porphyrin (magenta).

    Journal: Frontiers in Oncology

    Article Title: A novel auto-fluorescent porphyrin-lipid nanoparticle strategy for CTNNB1 gene silencing in hepatocellular carcinoma

    doi: 10.3389/fonc.2026.1779803

    Figure Lengend Snippet: Porphyrin-LNP synthesis and characterization. (A) Porphyrin-LNPs were synthesized via microfluidic rapid mixing. (B) . Cryo-TEM image of porphyrin-LNP (scale bar = 100 nm). (C) Size distribution of porphyrin-LNP-si-Scramble and (D) porphyrin-LNP-si-CTNNB1 measured by dynamic light scattering (DLS). (E) Confocal imaging of FAM-siRNA delivery by porphyrin-LNP. Hep3B and HepG2 cells were treated with porphyrin-LNP encapsulating FAM-siRNA (200 nM, ~2 μM porphyrin) for 6 h. Confocal images show FAM-siRNA (green) and porphyrin (magenta).

    Article Snippet: The Hep3B (HB-8064) and HepG2 (HB-8065) human liver cancer cell lines were obtained from the American Type Culture Collection (ATCC) and tested negative for mycoplasma contamination.

    Techniques: Synthesized, Imaging

    Validation of CTNNB1 knockdown efficiency using porphyrin-LNP-siRNA. Downregulation of CTNNB1 expression after transfection of porphyrin-LNP-siRNA targeting CTNNB1 in (A) Hep3B and (B) HepG2 cells. **** p < 0.0001; one-way ANOVA test. Reduced colony formation after transfection of porphyrin-LNP-siRNA targeting CTNNB1 in (C) Hep3B and (D) HepG2 cells. * p < 0.05, *** p < 0.001, one-way ANOVA test.

    Journal: Frontiers in Oncology

    Article Title: A novel auto-fluorescent porphyrin-lipid nanoparticle strategy for CTNNB1 gene silencing in hepatocellular carcinoma

    doi: 10.3389/fonc.2026.1779803

    Figure Lengend Snippet: Validation of CTNNB1 knockdown efficiency using porphyrin-LNP-siRNA. Downregulation of CTNNB1 expression after transfection of porphyrin-LNP-siRNA targeting CTNNB1 in (A) Hep3B and (B) HepG2 cells. **** p < 0.0001; one-way ANOVA test. Reduced colony formation after transfection of porphyrin-LNP-siRNA targeting CTNNB1 in (C) Hep3B and (D) HepG2 cells. * p < 0.05, *** p < 0.001, one-way ANOVA test.

    Article Snippet: The Hep3B (HB-8064) and HepG2 (HB-8065) human liver cancer cell lines were obtained from the American Type Culture Collection (ATCC) and tested negative for mycoplasma contamination.

    Techniques: Biomarker Discovery, Knockdown, Expressing, Transfection